四川南部珙县早侏罗世一新蜥脚类恐龙   总被引：3，自引：0，他引：3  

何信禄  王长生  刘尚忠  周凤云  刘图强  蔡开基  代兵 《四川地质学报》1998,(1)

简介在珙县下侏罗统自流井组东岳庙段中,发现的原始蜥脚类恐龙化石的一新属种石碑珙县龙。成年个体长约１４ｍ。化石材料相当丰富,除头骨不完全外,其他部分乃至全部均保存完好。它是早期的原始的蜥脚类,但又和蜥脚类有不少共同点。它对研究蜥脚类的起源和进化,具有重要意义  相似文献   

应用免疫荧光技术对三种赤潮藻的研究          下载免费PDF全文

孙宁波  隋正红  包振民  张冬宝 《海洋学报》2008,30(6):132-139

为探索和建立免疫学结合荧光技术对赤潮生物的定性、定量检测,制备了3种粒径不同的赤潮藻——赤潮异弯藻(Heterosigma akashiwo)、共生甲藻(Symbiodinium sp)、链状亚历山大藻(Alexandrium catenella)的多克隆抗体,并采用封闭吸附处理以提高抗体的特异性;采用间接酶联免疫法检测了抗体的效价;结果表明未经吸附处理的赤潮异弯藻、共生甲藻的效价分别为1:41 000,1:18 000以上,显示是高亲合力的抗体;链状亚历山大藻抗体的效价达到1:3 500,相比另两种藻的效价较低;吸附处理后的3种多克隆抗体的特异性明显提高,而效价有所下降。3种多克隆抗体与各自藻细胞均有很强的结合力,赤潮异弯藻和共生甲藻的荧光信号均匀分布于细胞表面,链状亚历山大藻细胞的荧光信号在细胞表面分布不均匀,主要集中在腹部横沟内。制备的多克隆抗体只与自身藻细胞结合,与另外2种藻细胞无交叉反应。链状亚历山大藻抗体只与自身藻细胞结合,与塔马亚历山大藻无交叉反应,可作为同属内不同种的鉴别。结合了免疫学和荧光技术,具有特异、灵敏、直观和简便的优点,并且费用低,对于开拓赤潮准确定性、定量检测方法和技术具有重要价值。  相似文献   

Aequorea taiwanensis n. sp. (Hydrozoa, Leptomedusae) and mtCOI sequence analysis for the genus Aequorea     

ZHENG Lianming  LIN Yuanshao  LI Shaojing  CAO Wenqing  XU Zhenzu  HUANG Jiaqi 《海洋学报(英文版)》2009,28(1):109-115

Aequorea taiwanensis, a new hydrozoan species from the Taiwan Strait was described using morphological and molecular characteristics. Both morphological and mitochondrial cytochrome oxidase subunit I (mtCOI) data supported A. taiwanensis n. sp. as a valid species. Sequence divergence and genetic distance of A. taiwanensis n. sp., A. papillata and A. conica were analysed based on the mtCOI gene sequences. The mtCOI sequences from these three species of the genus Aequorea showed high variation frequency, with sequence divergences ranging from 9.10% to 11.9%, and pairwise genetic distances ranging from 0.097 to 0.130. MtCOI sequence analysis provided diagnostic molecular systematic characteristics for accurate identification and discrimination of the species of Aequorea or their populations, and will be used to resolve evolutionary relationships among them.It was suggested that 10%—20% pairwise mtCOI sequence differences indicated the specieslevel divergence among congeneric species in the Hydromedusae.  相似文献   

富含古罗糖醛酸的褐藻多糖生产菌株的构建          下载免费PDF全文

胡斌  韩峰  林育姿  宫倩红  于文功 《海洋科学》2008,32(1):14-18

为获得古罗糖醛酸(Guluronate)含量高的细菌胞外褐藻多糖,利用PCR从海洋细菌Pseudomonassp.QDA中克隆了其甘露糖醛酸C-5差向异构酶基因(algG),连接入质粒pMF 54Km,构建了重组表达载体pMF54 Km-algG。利用三亲接合法将pMF54 Km-algG转入菌株QDA中,获得algG过量表达重组菌株QDA-G1。H-NMR测定结果表明,QDA-G所产的褐藻多糖中β-D-甘露糖醛酸(M)与它的C-5差向异构体α-L-古罗糖醛酸(G)的比值为0.38,G的质量分数达到74.2%,比野生菌株QDA提高了26.4%。且重组菌株遗传稳定性良好,连续传代20代后,M/G的比值无明显变化。  相似文献   

拮抗香蕉枯萎病菌海洋细菌的筛选和鉴定   总被引：3，自引：0，他引：3  

黄瑶  杨耿周  张雅娟  何秋韵  林巧玲  易润华 《广东海洋大学学报》2009,29(6):72-77

从93份中国南部海域近海海泥样品中分离出1800多株海洋细菌,以对峙培养法,测定海洋细菌对香蕉枯萎菌的拮抗作用,再以琼脂划线培养法(agar streak)测定菌株的抑菌活性,结果表明:约55.6%的菌株对香蕉枯萎菌有一定的拮抗作用,其中58株菌株抑制香蕉枯萎菌生长所形成的抑菌带宽度大于10.0mm,抑菌作用较好,复筛后其中13个菌株形成的抑菌带宽在15.0mm以上。经反复转接和对峙培养,筛选出拮抗效果好、抑菌活性稳定的菌株TC-1,初步鉴定为芽孢杆菌属的细菌,TC-1对14种病原微生物都有较好的抑菌效果。  相似文献   

High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A          下载免费PDF全文

Ornella Affinito  Nikos Andreakis  Luigi Caputi  Rita Marino  Raimondo Pannone  Paolo Sordino  Gabriele Procaccini 《Marine Ecology》2015,36(4):1230-1243

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.  相似文献   

中国南海一株固氮类芽孢杆菌的筛选和分离鉴定   总被引：1，自引：0，他引：1  

张丽红  刘艳芳  胡青平 《海洋科学》2015,39(3):100-105

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。  相似文献   

盐田嗜盐单胞杆菌的分离鉴定及其PHB 积累的研究     

隋丽英  张娇娇  许高超  邓元告 《海洋科学》2015,39(5):16-20

微生物聚合物聚-?-羟基丁酸酯(PHB)具有良好的生物降解性、生物相容性和热塑性,在环保、医药和农业领域具有广阔的应用前景。高PHB积累菌株的获得及其发酵条件的研究具有很好的实用价值。作者从渤海湾日晒盐场盐田卤水中分离出一株杆状细菌,经16S r DNA序列测序比对,确定为盐杆菌属(Halomonas),命名为Halomonas 100-16-2,其最适生长盐度为50~100,最适生长p H为7~8,属中度嗜盐菌。在酸水解酪蛋白(7.5 g/L)的基础培养基中分别添加0、10、20和30 g/L葡萄糖,研究盐度100条件下不同葡萄糖添加量对Halomonas 100-16-2生长和PHB含量的影响。结果表明,随着葡萄糖浓度的增加,该菌株的生长和PHB积累显著提高。葡萄糖质量浓度为30 g/L时,细胞干质量达8.60 g/L,PHB含量达到5.57 g/L(即达到细胞干质量的65%)。本研究为渤海湾盐田微生物资源的开发和利用提供了有价值的基础数据。  相似文献   

辽宁西部早白垩世朝阳翼龙科 (Chaoyangopteridae)化石一新种     

姬书安  张立军  路芳 《地质学报》2023,97(6):1723-1740

中国辽宁西部早白垩世翼龙类化石非常丰富,其上部的九佛堂组翼龙类组合以含有丰富的无齿的古神翼龙科、朝阳翼龙科等进步类型而与下部的义县组翼龙类组合相区别。本文描述了辽宁西部建昌盆地九佛堂组一新的大型翼龙类化石,其以上下颌无齿、吻端指数为3.7、前颌骨背支细长且封闭鼻眶前孔背缘、很大的鼻眶前孔后端超过上下颌关节位置、轭骨的上颌骨突基部宽大而应被归入朝阳翼龙科(Chaoyangopteridae)神州翼龙属(Shenzhoupterus)。其以较大的体形(翼展2.05 m)、平直的上下颌咬合面、轭骨的眶后骨突较泪骨突长且基部略宽、第4～7颈椎长度依次减小等特征,区别于朝阳神州翼龙(Shenzhoupterus chaoyangensis),而被命名为三亚神州翼龙(新种)(Shenzhoupterus sanyainus sp. nov.)。这是辽宁西部早白垩世翼展最大且不具牙齿的翼龙类属种,它的发现丰富了九佛堂组翼龙类组合内容,对认识朝阳翼龙科的骨骼形态与生态习性亦具有积极意义。  相似文献   

Survey of historical New Zealand shellfish samples for accumulation of gymnodimine     

David J. Stirling 《新西兰海洋与淡水研究杂志》2013,47(4):851-857

In 1994 a major biotoxin event occurred along the east coast of the South Island, New Zealand. Gymnodimine, a unique bioactive spiroimine, was isolated and characterised from Foveaux Strait dredge oysters (Tiostrea chilensis = Ostrea chilensis) collected during this outbreak. This study reports the results of liquid chromatographic‐mass spectrometric analysis for gymnodimine in 217 samples from eight species of shellfish over the years 1993–99. Of these samples 155, covering six species of shellfish, contained detectable gymnodimine with a range in concentration from 14.8 to 23 400 μg/kg. Gymnodimine‐containing shellfish occurred at 37 of the 63 sites sampled from around New Zealand. This study demonstrates that accumulation of gymnodimine is not limited to T. chilensis and can occur in other shellfish species over much of New Zealand. Gymnodimine is a possible cause of the numerous historical biotoxin screen‐positive results.  相似文献